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凝胶柱色谱(gel column choromatography)英文PPT 13页

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  • 2022-05-26 16:31:05 发布

凝胶柱色谱(gel column choromatography)英文PPT

  • 13页
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ExperimentMethodsMasseyUniversity GelcolumnchromatographySeparationScience ContentDefinitionWhentouseMechanismAdvantages&disadvantagesAnexample DefinitionofgelcolumnchromatographyGelcolumnchromatographyisakindofseparationmethodthatdependsontheprincipleofdifferentsizesofmoleculescanbeseparatedbytheporouspolymerbead,smallmoleculesenterthebeadwhilelargemoleculescan"tenterthebead,andmedium-sizemoleculespartiallyenterthebead. Whentouse?SeparationinChemistryAnalyticalChemistryPhytoChemistryNaturalMedicinalChemistryBiochemistryOtherfields MechanismStationaryphase:Thephasethatisstationaryandcanseparateandpreservetheresearchsampleinchromatography.Mobilephase:Thesubstancesthatcarrytheanalytesandmoveforwardthroughthestationaryphaseinchromatography. Advantages&disadvantagesAdvantages:1.Simple2.Goodseparationeffecttowardspolymericsubstances3.Maintainthebiologicalproperties4.Estimatedmolecularweightofthebiologicalmacromolecules Advantages&disadvantagesDisadvantages:1.Timeconsuming2.Needtobetakencareofcarefully3.Preliminaryseparation AnexampleSmallmoleculesLargemoleculesMidium-sizemolecules ReferencesKremmer,T.(1979).Gelchromatography:theory,methodology,applications/byTiborKremmerandLászlóBoross.London;NewYork:Wiley.

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